ISOLATION AND FULL CHARACTERISATION OF TWO STEROL GLUCOSIDES FROM TEUCRIUM BARBEYANUM ASCHERS
DOI:
https://doi.org/10.53555/eijas.v6i2.101Keywords:
Teucrium barbeyanum, sterol glucosides, sitosterol-3-O-β-Dglucoside, clerosterol-3-O-β-D-glucoside, 1D and 2D NMRAbstract
The genus Teucrium is used in folk medicine for a long time as hypoglycemic and hypolipidemic agents, and showed a wide spectrum of interesting biological activities. In this study, the aerial parts of Teucrium barbeyanum which collected from Ras El-Hilal, Libya were subject of extraction with different organic solvents, followed by extensive columns chromatography and crystallization using various solvent systems in order to isolate compounds. Dichloromethane extract afforded two sterol glucosides which successfully identified as sitosterol-3-O-β-D-glucoside and the rare compound, clerosterol-3-O-β-D-glucoside. The protons, carbons and OHgroups were fully assigned by means of spectroscopic techniques including NMR (1H, 13C, DEPT-135, 90 and Q, COSY, HSQC and HMBC) and IR, and Electron Impact Mass Spectrometry.
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