ANTIBACTERIAL STUDY OF CHLORELLA VULGARIS ISOLATED FROM FRESH WATER
DOI:
https://doi.org/10.53555/eijas.v5i3.115Keywords:
Antimicrobial, Identifications, Isolation, Chlorella vulgarisAbstract
Chlorella vulgaris is growing in either fresh water or sea water. It can provide various other nutrients including proteins, minerals, vitamins, and antioxidants. World production of consumable algae and algae products to be used as foods and medicines have reached thousands of tons per year. In this study, Chlorella vulgaris was collected and isolated from freshwater. The extracted of chlorella vulgaris assay was tested to investigate its efficiency against four bacterial strains (Achromobacter sp (S1), Staphylococcus sp (S2) Escherichia coli (S3), Shigella dysenteriae (S4)), and was determined by disk diffusion method. Different concentration extracts from the microalgae Chlorella vulgaris (25, 50, 75 and 100%) were used. Results showed that the 75% of the extract was highly significant against Escherichia coli and followed by concentration 25% against Achromobacter sp, however, the lowest significant against Staphylococcus sp at the concentration 100%. The antimicrobial activity of the Chlorella vulgaris extract was higher than the antibiotics used against the testes microorganisms.
References
. Tandeau-de-Marsac, HJ. Adaptation of cyanobacteria to environmental stimuli: new steps towards molecular mechanisms. FEMS Microbiology Reviews. 1993; 104: 119190.
. Davey, H.M.; Kell, D.B. Flow cytometry and cell sorting of heterogeneous microbial populations: The importance of single-cell analyses. Microbiol. Rev. 1996, 60, 641–696.
. K. S. Diker, M. Akan, G. Hascelik and M. Yurdakok, „The bactericidal activity of te against Campylobacter jejuni and Campylobacter coli. Lett Appl Microbiol“, vol. 12, (1991), pp. 34-54.
. J. A. Findlay and A. D. Patil, „Antibacterial constituents of the diatom Navicula delogne“, Journal of Natural Products, vol. 47, (1984), pp. 815-818.
. Tramper J, Battershill C, Brandenburg W, Burgess G, Hill R, Luiten E, Müller W, Osinga R, Rorrer G, Tredici M, Uriz M, Wright P, Wijffels R. What to do in marine biotechnology? Biomolecular Engineering. 2003; 20: 467-471.
. Gonzalez del Val, G. Platas and A. Basilio, „Screening of antimicrobial activities in red, green and brown macroalgae from Gran Canaria (Canary Islands, Spain)“, Int.Microbiol, vol. 4, (2001), pp. 35-40.
. Harborne, „Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis“, 3rd Edition. Chapman & Hall Pub. London, UK, (1998).
. M. Herrero, E. Ibañez, A. Cifuentes, G. Reglero and S. Santoyo, „Dunaliella salina microalga pressurized liquid extracts as potential antimicrobials“, Journal of Food Protection, vol. 69, (2006), pp. 2471-2477.
. Chattopadhyay I, Biswas K, Bandyopadhyay U, Banerjee RK (2004).Turmeric and curcumin: Biological actions and medicinal applications. Curr. Sci. 87: 44-53.
. Issa A.A. (1999): Interference of glyphosate with the shikimate pathway by cyanobacteria in chemostat culture. Microbios.100, 47-52.
. Nichols, H. W. (1973). In Handbook of Phycological Methods, Ed. J. R Stein, pp 16-17. Camb. Univ. Press. (R. R. L. Guillard, personal communication).
. Jurgensen, M.F. and C.B. Davey, 1968. Nitrogen fixating blue-green algae in acid forest and nursery soils. Can. J. Microb., 14: 1179-1179
. Rippka R, Deruelles J, Waterbury J, Herdman M, Stanier R (1979). Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol., 111: 1-6.
. H. Al-Wathnani, Ismet Ara*, R. R. Tahmaz, T. H. Al-Dayel and M. A. Bakir (2012) Bioactivity of natural compounds isolated from cyanobacteria and green algae against human pathogenic bacteria and yeast . Journal of Medicinal Plants Research Vol. 6(18), pp. 3425-3433.
. L. Drago, B. Mombelli, deVecchi E., M.C. Fassina, L. Tocalli, M.R. GismondoIn vitro antimicrobial activity of propolis dry extract J. Chemotherapy, 12 (2000), pp. 390-395.
. Bauer, A.W., Kirby, W.M., Sherris, J.C. and Turck, M., 1996. Antibiotic susceptibility testing by a standardized single disk method. American Journal Clinical Pathology, 45:493-496.
. Suresh K (2009): Introduction to algae .Campus Books international PP 49.
. B. Metting and J. W. Pyne, „Biologically active compounds from microalgae“, Enzyme and Microbial Technology, vol. 8, (1986), pp. 386-394.
. P. S. Syed Shabudeen, M. Soundrarajan and P. Indumathi, “Algae biomass growth kinetic study in wasteWater medium using spectroscopic analysis”, Journal of Environmental Research And Development, vol. 7, no. 4A, April-June (2013)
. Jyotrimayee p, Sachidandndda D and Bastanta K.D (2014): Antibacteria activity of fresh water algae.academic journals Vol8 (32) PP809.818..
. Yingying S, Changhai W, Jing C. Growth inhibition of eight species of microalgae by growth inhibitor from the culture of Isochrysis galbana and its isolation and identification. Journal of Applied Phycology. 2008; 20:315-321.
. Wu JT, Kuo-Huang LL, Lee J. Algicidal effect of peridinium bipes on Microcystis aeruginosa. Current Microbiology. 1998;37:257-261
. Santoyo S, Rodríguez-Meizoso I, Cifuentes A, Jaime L, García-Blairsy Reina G, Señorans FJ, Ibáñez E. Green processes based on the extraction with pressurized fluids to obtain potent antimicrobials from Haematococcus pluvialis microalgae. LWT – Food Science and Technology. 2009; 42:1213-1218.
. Gianluca Pane, Gabriele Cacciola, Elisabetta Giacco, Gian Luigi Mariottini, and Erika Coppo (2015) Assessment of the Antimicrobial Activity of Algae Extracts on Bacteria Responsible of External.
Downloads
Published
Issue
Section
License
Copyright (c) 2019 EPH - International Journal of Applied Science (ISSN: 2208-2182)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
