PESTICIDE POLLUTION AND AQUATIC HEALTH: CYHALOTHRIN TOXICITY IN CYPRINUS CARPIO
DOI:
https://doi.org/10.53555/eijas.v9i4.196Keywords:
Cyprinus carpio, lambda-cyhalothrin, oxidative stress, neurotoxicity, histopathologyAbstract
Synthetic pyrethroid pesticides, with lambda-cyhalothrin (LCH) being the most frequently used, have been applied extensively in agriculture, but there is much concern about their possible ecotoxicological impacts on aquatic ecosystems. This investigates the sublethal paraphernalia of LCH on the bio-chemical, enzymatic, haematological, and histopathological parameters of Cyprinus carpio, a sentinel freshwater species. Under semi-static conditions, fish were exposed to a LCH effective concentration (1.6 µg/L) for 1, 5, 10, 15, and 20 days. Physiological response was assessed in multiple tissues, including the liver, gill, kidney, muscle, intestine, ovary, testis, and blood. Subsequently, it was found that there was time time-dependent decline in soluble, structural, and total protein concentrations with increased free amino acids, protease activity, indicating disturbed protein metabolism as determined by biochemical assays. Elevated acetylcholine levels and inhibited acetylcholinesterase activity were evidenced as neurotoxic effects. Osmoregulatory dysfunction was manifested as ionic imbalance and suppressed activities of Na⁺/K⁺, Ca²⁺, and Mg²⁺ ATPases. It was found that antioxidant enzymes activities (catalase, SOD, GPx, GST) were reduced while malondialdehydes levels were increased, which indicates oxidative stress. There were signs of hepatic injury in the serum AST, ALT, and ALP. Haematological analysis showed marked decreases in RBC and hemoglobin as well as increased WBC counts consistent with anemia and immune stress. Biochemical findings were validated by histopathological changes of necrosis and cellular degeneration, not only in the kidneys, but also in other organs. However, these results underscore the fact that even sublethal exposure to LCH can result in systemic toxicity in fish and thus present serious ecological risks. Ensuring environmentally friendly ‘preparedness’ and scrutinised pesticide regulation is vital to protecting aquatic life from pesticide exposure, the study concluded.
References
Altun, S., Özdemir, S., & Arslan, H. (2017). Histopathological effects, responses of oxidative stress, inflammation, apoptosis biomarkers, and alteration of gene expressions related to apoptosis, oxidative stress, and the reproductive system in chlorpyrifos-exposed common carp (Cyprinus carpio L.). Environmental Pollution, 230, 432-443.
Andem, A. B., Ibor, O. R., Joseph, A. P., Eyo, V. O., & Edet, A. A. (2016). Toxicological evaluation and histopathological changes of synthetic pyrethroid pesticide (Cypermethrin) exposed to African Clariid mud catfish (Clarias gariepinus) fingerlings. Journal of Toxicological and Pharmacological Research. 2016b, 8(5), 360-367.
Banaee, M. (2013). Physiological dysfunction in fish after insecticide exposure. In Insecticides-Development of safer and more effective technologies. IntechOpen.
El-Bendary, H. M., Negm, S. E., Saleh, A. A., Kady, M. M., & Hosam Eldeen, F. A. (2010). HISTOPATHOLOGICAL CHANGES ASSOCIATED WITH EXPOSURE OF MALE MICE TO PYRETHROID PESTICIDE (LAMBDA-CYHALOTHRIN). Journal of Plant Protection and Pathology, 1(9), 697-710.
Ensibi, C., Pérez-López, M., Rodríguez, F. S., Míguez-Santiyán, M. P., Yahya, M. D., & Hernández-Moreno, D. (2013). Effects of deltamethrin on biometric parameters and liver biomarkers in common carp (Cyprinus carpio L.). Environmental Toxicology and Pharmacology, 36(2), 384-391.
Jaffer, N. S., Rabee, A. M., & Al-Chalabi, S. M. (2017). Biochemical and hematological parameters and histological alterations in fish Cyprinus carpio L. as biomarkers for water pollution with chlorpyrifos. Human and Ecological Risk Assessment: An International Journal, 23(3), 605-616.
Korkmaz, N., Cengiz, E. I., Unlu, E., Uysal, E., & Yanar, M. A. H. M. U. T. (2009). Cypermethrin-induced histopathological and biochemical changes in Nile tilapia (Oreochromis niloticus), and the protective and recuperative effect of ascorbic acid. Environmental toxicology and pharmacology, 28(2), 198-205.
Kumar, A., Sharma, B., & Pandey, R. S. (2012). Assessment of stress in response to pyrethroid insecticides, λ-cyhalothrin and cypermethrin, in a freshwater fish, Channa punctatus (BLOCH). Cellular and Molecular Biology, 58(1), 153-159.
Neelima, P., Rao, K. G., Rao, N. G., & Jammu, C. S. R. (2015). Enzymatic alterations as biomarkers of cypermethrin (25% EC) toxicity in a freshwater fish, Cyprinus carpio (Linn.). Int J Fish Aquat Stud, 3(1), 149-158.
Orun, I., Selamoglu, Z., Gulhan, M. F., & Erdogan, K. (2014). Role of propolis on biochemical and hematological parameters of Oncorhynchus mykiss exposed to cypermethrin. Journal of Survey in Fisheries Sciences, 1(1), 21-35.
Richterová, Z., Máchová, J., Stará, A., Tumová, J., Velíšek, J., Ševčíková, M., & Svobodová, Z. (2014). Effects of Cyhalothrin‐Based Pesticide on Early Life Stages of Common Carp (Cyprinus carpio L.). BioMed Research International, 2014(1), 107373.
Srivastava, P., Singh, A., & Pandey, A. K. (2016). Pesticides toxicity in fishes: biochemical, physiological and genotoxic aspects. Biochemical and cellular archives, 16(2), 199-218.
Stoyanova, S., Yancheva, V., Iliev, I., Vasileva, T., Bivolarski, V., Velcheva, I., & Georgieva, E. (2016). Biochemical, histological, and histochemical changes in Aristichthys nobilis Rich. liver exposed to thiamethoxam. Periodicum biologorum, 118(1).
Velisek, J., Svobodova, Z., & Machova, J. (2009). Effects of bifenthrin on some haematological, biochemical and histopathological parameters of common carp (Cyprinus carpio L.). Fish physiology and biochemistry, 35, 583-590.
Velmurugan, B., Selvanayagam, M., Cengiz, E. I., & Unlu, E. (2007). Histopathology of lambda-cyhalothrin on tissues (gill, kidney, liver, and intestine) of Cirrhinus mrigala. Environmental Toxicology and Pharmacology, 24(3), 286-291.
Yasser, A. G. (2012). Haematological and micronuclei alterations in common carp Cyprinus carpio exposed to cypermethrin. J Thi-Qar Sci, 3(3), 37-46.
Yekeen, T. A., Fawole, O. O., & Bakare, A. A. (2013). Evaluation of toxic effects of lambdacyhalothrin on the haematology and selected biochemical parameters of African catfish Clarias gariepinus. Zoology and Ecology, 23(1), 45-52.
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