HARNESSING MICROALGAL DIVERSITY FROM DHOSI HILL, MAHENDERGARH, HARYANA, INDIA FOR RENEWABLE BIODIESEL APPLICATIONS
DOI:
https://doi.org/10.53555/8za5j306Keywords:
Microalgae, 18s rRNA, molecular identification, feedstocks, biodieselAbstract
Microalgae are recognized as hopeful sustainable feedstocks for biodiesel production due to their fast growth rate, high photosynthetic efficiency, and ability to accumulate considerable lipid content. The present study focuses on the isolation, purification, and molecular identification of freshwater microalgae from Shiv Kund Dhosi Hill, Mahendergarh, Haryana, India, and the evaluation of their biodiesel production potential. From the water source one algae was isolated and named as MKI01, it was successfully established in sterile culture. Biodiesel extraction using a solvent-based method revealed that MKI01 yielded a higher lipid-derived biodiesel output, producing approximately 10.0 ± 0.1 mL from 20 g of dried biomass. Molecular identification using 18S rRNA gene sequencing confirmed MKI01 was Chlorella sp. showing 100% similarity with reference sequences in the NCBI database. Phylogenetic analysis further supported the taxonomic placement. The results focous on Chlorella sp. from the Dhosi Hill region as a promising candidate for biodiesel production.
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