Comparison the Antibacterial and Antibiofilm Activity of Citrullus Colocynthis Seeds Aqueous Extract against the Bacteria Isolated from human and sheep Oral cavity
DOI:
https://doi.org/10.69980/txfpm975Keywords:
Citrullus colocynthis seed extract, anti-bacterial, anti-biofilm, oral cavity, MICAbstract
The current study was comparison between the bacteria isolated from oral cavity of animal and human against Citrullus colocynthis seeds extract, bacterial isolates were obtained from Al-Muthanna University, College of Veterinary Medicine, the isolates were isolated and from sheep's mouth that located in the Salman area, same isolates were isolated from human's mouth such as Raoultella ornithinolytica, Citrobacter freundii, Pseudomonas aeruginosa, Enterobacter cloacae, The MIC of C.colocynthis seeds extract was ranged from 100 to 66 mg/ml ,while the antibacterial activity results showed generally the isolates from sheep was more resistance than human with significant difference at p ≤ 0.05 in different concentration, However the higher antibacterial activity noted in 400 mg/ml , in addition E.cloacae showed more susceptibility toward the extract in 200 mg/ml, 300 mg/ml and 400 mg/ml, the anti biofilm formation activity showed the bacteria from sheep had biofilm formation stronger than human's isolates with significant differences at p≤0.05 but after exposure to the extract the biofilm formation was decreased with no significantly between human and sheep isolates at p≤0.05, from the other side the findings reported a there was a significant difference before and after the treatment at p ≤ 0.05 in both animal's isolates and human's isolates. the C. colocynthis seedsextract showed antibacterial and antibioilm formation activity against the isolates from human and sheep source but the bacteria from sheep showed resistance to the extract higher than the bacteria from human source .
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