SURVIVAL OF AZOSPIRILLUM BRASILENSE UNDER DIFFERENT MOISTURE CONTENT LEVELS
DOI:
https://doi.org/10.53555/eijas.v5i4.111Keywords:
Azospirillum brasilense, Sterilized, Bulk soil, DernaAbstract
The survival and proliferation of free and alginate immobilized, Azospirillum brasilense in a bulk soil collected from Derna region at different moisture content levels (10%, 40% and 60 %). A reduction of cell number in the experimental units (sterile or non-sterile soil) for all the tested moisture levels was observed after 15 days after the inoculation. Nevertheless, different parameters among the level of moisture content could be observed in the reduction of the population .soil-sterilization did not affect Azospirillum survival and proliferation, and results presented in results show that Azospirillum brasilense total content was not affected by the sterility of the soil. In general, Azospirillum brasilense strain isolated from Derna soil survived well and for long period (75day) in bluk soil (without plant roots). In this study the presence of moisture in the soil as well as the soil- type of Derna which is rich in organic matter maintained Azospirillum brasilense population of a level high enough to be detected 75 days after inoculation.
References
. Bashan, Y., (1999). Interactions of Azospirillum spp. in soils: a review. Biol. Fertil. Soils, 29: 246–256.
. Harris, J.M., Lucas, J.A., Davey, M.R., and Lethbridge, G., (1989). Establishment of Azospirillum inoculants in the rhizosph of winter wheat. Soil Biol. Biochem. 21:59-64.
. Oliveira, R.G.B., and Drozdowicz, A., (1988). Are Azospirillum bacteriocins produced and active in soil? In: Klingmüller W (ed) Azospirillum IV: genetics, physiology, ecology. Springer, Berlin Heidelberg New York, pp 101-108.
. Olivera, A., Canuto, E., and Baldani, J., (2004). Survival of endophytic bacteria in soil under different moisture levels. Brazil. J. Microbiol. 36: 295-299
. Albrecht, S.L., Gaskins, M.H., Milam, J.R, Schank S.C., and Smith, R.L., (1983). Ecological factors affecting survival and activity of Azospirillum in the rhizosphere. Experientia. Suppl. 48:138148.
. Smith, R.L., Schank, S. C., Milam, J. R., and Baltensperger. A.A., (1984). Responses of Sorghum and Pennisetum species to the N2- fixing bacterium Azospirillum brasilense. Appl Environ Microbiol 47:1331-1336.
. Shaban, G., and El-Komy, H., (2000). Survival and proliferation of alginate encapsulated Trichoderma in Egyptian soil. Mycopath. 151: 139-146.
. El-Komy, H., (2001). Survival of and wheat – root colonization by alginate encapsulated Herbaspirillum spp. Folia. Microbiol. 46: 25-30.
. El-Komy., H. (2005). Coimmobilization of Azospirillum lipoferum and Bacillus megaterium for successful phosphorus and nitrogen nutrition of wheat plants. Food Technol. Biotechnol. 43: 19-27.
. Rodrigues-Caceres, E., (1982). Improved medium for isolation of Azospirillum spp. Appl. Environ. Microbiol. 44: 990-991.
. Bashan, Y,. Puente, M.E., Rodriguez-Mendoza, M.N.,Toledo, G., Holguin, G., Ferrera-Cerrato, R., and Pedrin,S., (1995a). Survival of Azospirillum brasilense in the bulk soil and rhizosphere of 23 soil types. App.Environ. Microbiol. 61:1938-1945.
. Bashan, Y. and Vazquez, P., (2000) Effect of calcium carbonate, sand, and organic matter levels on mortality of five species of Azospirillum in natural and artificial bulk soils. Biol .Fertil. Soils, 30 (5-6):450-459, ISSN: 0178-2762.
. El-Katatny, M.S., El-Komy, H.M, Shaban, G.M., Hetta, A.M., and El-Katatny, M.H., (2004). Effect of benomyl on chitinase and 0-1,3--glucanase production by free and alginate encapsulated Trichoderma harzianum. Food Technol. Biotechnol. 42: 8388.
. Vandenhove, H., Merckx, R., van Steenbergen, M., and Vlassak, K., (1993). Microcalorimetric characterization, physiological stages and survival ability of Azospirillum bra silense. Soil Biol Biochem 25:513-519.
. Heijnen, C., Hok-A- Hin, J., and Van Elases, J., (1993). Root colonization by Pseudomonas fluresence inoculated into soil with bentonite. Soil Biol. Biochem. 25: 239-246.
. Heijnen, C., Van Elase, J., and Van Veen, J., (1988). Dynamics of Rhizobium leguminosarum introduced into soil. Soil Biol. Biochem. 20: 483- 488.
. Heijnen, C.E., and Van Veen,J.A., (1991). A determination of protective microhabitats for bacteria introduced into soil.FEMS Microbiol Ecology, 85:73-80.
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