SCREENING OF DIFFERENT ISOLATES OF AZOSPIRILLUMFOR GROWTH AT DIFFERENT SALT (NACL) CONCENTRATIONS

Authors

  • Amal A.A. Taher Botany Department, Faculty of Science, Omar El-Mokhtar University, El -Beyda-Libya
  • Nagah S. A. Abubaker Botany Department, Faculty of Science, Omar El-Mokhtar University, El -Beyda-Libya
  • Hesham M. A. El-Komy Botany Department, Faculty of Science, Omar El-Mokhtar University, El -Beyda-Libya

DOI:

https://doi.org/10.53555/eijas.v4i4.124

Keywords:

Azospirillum, salt, concentration

Abstract

Azospirillum was abundant in the rhizosphere of halophytes collected from El- Thaliga salt marsh near Benghazi, Libya. More than twenty five isolated of Azospirillum were isolated from rhizosphere by enrichement method using DN semisolid medium with or without salt. After several transfer for purification, 13 isolates were screened to select the most efficient strains in a microcosms experiment with wheat. According to the cultural, morphological and biochemical experiments Azospirillum isolated were belonging to A. brasilense and isolated was belonging to A. lipoferum, Azospirillum isolates were screened for growth at different NaCl concentration in liquid DN medium. 

References

. Bashan, Y and Holguin, G (1997): Azospirillum plant relationships environmental and physiological advances (1990-1996); Can. J. Microbiol. 43: 103-121.

. Bashan, Y.; Holguin, G. and de- Bashan, L. (2004): Azospirillum- plant relationships, molecular, and environmental advances. Can. J. Microbiol. 50: 521-577.

. Bashan, Y.; Puente, M.E.; Rodriguez-Mendoza, M.N.; Toledo, G.; Holguin, G.; FerreraCerrato, R. and Pedrin, S. (1995): Survival of Azospirillum brasilense in the bulk soil and rhizosphere of 23 soil types. App.Environ. Microbiol. 61:1938- 1945.

. Beijerinck, M.W. (1901): Ueber ein Spirillum, Welches freien sticktoff binden kann Centralbl. Bakt. II Abt. 63 353-357.

. El-Komy, H. (2005): Coimmobilization of Azospirillum lipoferum and Bacillus megaterium for successful phosphorus and nitrogen nutrition of wheat plants. Food Technol. Biotechnol. 43: 19-27.

. Elkomy, H. and Abdel. wahab, A. (1998): Effect of simultaneous inoculation of Azospirillum and Rhizobium spp. On growt and nitrogen fixation of two legumes using 15Ndilution technique and the difference method. Acta. Microbiol. Pol. 47: 283-296.

. Kabir, M.; Chotte, J.L.; Rahman, M.; Bally, R. and Jocteur Monrozier, L. (1994): Distribution of soil fractions and location of soil bacteria in a vertisol under cultivation and perennial grass. Plant Soil 163: 243-255.

. Mayak, S.; Tirosh,T. and Glick, B.R. (2004): Plant growth promoting bacteria confer resistance in tomato and pepper plants to salt stress. Plant Physiol. Biochem. 167:650-656.

. Tarrand, J.J.; Kreig, N.R.; Döbereiner, J. (1978): A taxonomic study of the Spirillum lipoferum group, with a descriptions of a new genus, Azospirillum gen. nov. And two species, Azospirillum lipoferum (Beijerink) comb. nov. And Azospirillum brasilense sp. nov. Can. J. Microbiol. 24:967-980.

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Published

2018-12-27

Issue

Vol. 4 No. 4 (2018): EPH - International Journal of Applied Science ( ISSN: 2208-2182 )

Section

Articles

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Copyright (c) 2018 EPH - International Journal of Applied Science (ISSN: 2208-2182)

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