COMPARISON OF CEFOXITIN AND OXACILLIN DISKS DIFFUSION TEST FOR DETECTION OF MRSA STRAINS
DOI:
https://doi.org/10.53555/eijas.v2i4.136Keywords:
MRSA, Oxacillin DD test, Cefoxitin DD test, BD PhoenixBD PhoenixAbstract
It is very important for clinical microbiology laboratories to be able to detect MRSA strains accurately and reasonably fast, for the adjustment of antibiotic treatment, and for the implementation of infection control measures. Detection of the mecA gene or PBP2a is considered the gold standard for detecting MRSA. However, many laboratories throughout the world do not have the capacity to use molecular techniques to detect MRSA in routine clinical practice. This study evaluated the performance of the cefoxitin and oxacillin DD test in determining methicillin resistance in comparison to the BD Phoenix automated system (BD, Sparks, MD). A total of (359) S. aureus strains which were isolated from were included in this study. All isolates were tested for methicillin resistance by cefoxitin DD test and oxacillin DD test considering the BD Phoenix automated system as gold standard. Among the (359) S. auresus isolates 146 (40.7%) isolate were identified as MRSA by the BD Phoenix automated system (BD, Sparks, MD). Cefoxitin DD test showed 100% sensitivity and 100% specificity, while oxacillin DD test showed 97.5% sensitivity and 98.6% specificity. In a laboratory where it is not possible to carry out molecular method as a routine, cefoxitin disk diffusion test is a good surrogate marker for detecting methicillin resistance. It is far superior to most of the currently recommended phenotypic method. It is now an acceptable method for detection of MRSA by many reference groups including CLSI.
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