PLANT TISSUE CULTURE OF AN ENDANGERED MEDICINAL HERB BACOPA MONNIERI
DOI:
https://doi.org/10.53555/eijas.v10i1.173Keywords:
Medicinal plant, in-vitro cultivation, Endangered plantAbstract
Bacopa monnieri, a valuable medicinal herb, is endangered and is often propagated vegetatively. It is well-known for its versatility in applications. Bacopa monnieri ,a plant with strong roots in the Indian ancient Ayurvedic system, has long been known as an effective treatment. Commonly known as "Brahmi," this medicinal plant contains important phytopharmaceuticals substances.
Given its importance in treating a variety of disorders, producing Bacopa monnieri via in vitro cultivation is crucial. Aseptic cultures were produced and begun on Murashige and Skoog (MS) media enriched with 6-benzyl adenine (0.5μM). For shoot multiplication, a combination of BA, Kinetin (KIN), and Thidizuron (TDZ) was used. BA surpassed KIN and TDZ in terms of multiplication of shoots and elongation.MS medium with 2.5μM of BA resulted in the maximum shoot multiplication. Furthermore, the greatest shoot regeneration frequency was found on MS medium enriched with 5.0μM of BA and 1.0μM of α-naphthalene acetic acid (NAA).
This plant is suggested in the Indian Materia Medica (Bhavaprakasha Nighantu AD 1500) for treating a wide range of mental illnesses, including stress, poor cognition, lack of focus, sleeplessness, insanity, depression, psychosis, epilepsy, and Alzheimer's disease [1,2]. Commercially accessible extracts of B. monnieri have been shown to improve memory focus, cognitive development, and general brain function in young as well as older people. Furthermore, the plant has a long history of usage as a heart tonic and digestive aid in India and Pakistan, with reports of improved respiratory performance during bronchoconstriction. Clinical studies demonstrate the favorable benefits of bacopa-based compounds in the recovery of brain functions in children with diagnoses of attention deficit hyperactivity disorder (ADHD), as well as the augmentation of cognitive capacities in patients recovering from stroke.
It is also used in cosmetic industries as it contains essential oils, sterols, flavonoids, glycosides, as well as triterpenoid saponins. Two commercially available hair care formulations—Brahmi oil and soft extract—make use of Brahmi's positive characteristics. It is also classified as classified as a Rasayana in Ayurveda, is known for its ability to postpone indications of aging in the body, such as hair graying. Furthermore, Brahmi helps to alleviate mental weariness, which contributes to the preservation of a favorable body environment for healthy hair [3].
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