APPLICATION OF PHENOTYPIC AND GENOTYPIC STRATEGIES TO IDENTIFY PSEUDOMONAS AERUGINOSA

Authors

  • Mohamed Eswani1 Dept. of cell and human tissues culture research, biotechnology research Centre -Tripoli, Libya
  • Ashraf Naass National Nanoscience and Nanotechnology Project, biotechnology research center- Tripoli, Libya.
  • Nagib A. Elmarzugi Dept. of cell and human tissues culture research, biotechnology research Centre -Tripoli, Libya
  • Ahmed M Elmarghani Dept. of cell and human tissues culture research, biotechnology research Centre -Tripoli, Libya
  • Mohammed Elfgee Dept. of cell and human tissues culture research, biotechnology research Centre -Tripoli, Libya
  • Mohammed O Elbasir Dept. of cell and human tissues culture research, biotechnology research Centre -Tripoli, Libya

DOI:

https://doi.org/10.53555/eijas.v7i3.52

Keywords:

Pseudomonas aeruginosa, phenotype, genotype, RT-PCR

Abstract

A case study of patient presented with developed wound infection with Pseudomonas aeruginosa (leukocytosis). The strains of P. aeruginosa isolated from Sheffield General Northern Hospital (UK) and studied for phenotypic and genotypic identifications. This present study were conducted in Sheffield Hallam University (UK) and was aimed to evaluate the application of Phenotypic and Genotypic techniques to identify P. aeruginosa. Generally, the results of Real time PCR (RTPCR) revealed that the diagnosis viability was confirmed for P. aeruginosa. However, identification on the basis of phenotype by differences in types of growth on cetrimide and acetamide agar medium presents that both agars were conducive to the growth of P. aeruginosa. The strains of P. aeruginosa were also tested for antibiotic susceptibility to six different antibiotics, imipenem showed the greatest inhibition effect on the bacteria. It was observed that genetic techniques in accordance with phenotypic tests have facilitated to conduct a comprehensive characterization of P. aeruginosa strains obtained from a particular environment at a particular time. 

References

. BOTZENHART, Konrad and DÖRING, Gerd (1993). Ecology and epidemiology of Pseudomonas aeruginosa. In: Pseudomonas aeruginosa as an Opportunistic Pathogen. Springer, 1-18.

. PIRNAY, Jean-Paul, et al. (2009). Pseudomonas aeruginosa population structure revisited. PLoS one, 4 (11), e7740.

. TILLOTSON, JAMES R. and LERNER, A. MARTIN (1968). Characteristics of nonbacteremic Pseudomonas pneumonia. Annals of internal medicine, 68 (2), 295-307.

. MOORE, Nicholas M. and FLAWS, Maribeth L. (2011). Introduction: Pseudomonas aeruginosa. Clinical laboratory science, 24 (1), 41.

. KANG, Cheol-In, et al. (2003). Pseudomonas aeruginosa bacteremia: risk factors for mortality and influence of delayed receipt of effective antimicrobial therapy on clinical outcome. Clinical infectious diseases, 37 (6), 745-751.

. MURPHY, Timothy F., et al. (2008). Pseudomonas aeruginosa in chronic obstructive pulmonary disease. American journal of respiratory and critical care medicine, 177 (8), 853-860.

. Speert, D.P. (2002). Molecular epidemiology of Pseudomonas aeruginosa. Front. Biosci. 1 (7), e354-361.

. Kingsford, N.M.; Raadsma, H.W. (1995). Detection of Pseudomonas aeruginosa from ovine fleece washings by PCR amplication of 16S ribosomal RNA. Vet. Microbiol. 47, 61-70.

. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial testing (2006). 16th informational supplement M100-S16. Wayne, Pa: CLSI.

. HOIBY, Niels, et al. (1977). Pseudomonas aeruginosa infection in cystic fibrosis. Diagnostic and prognostic significance of Pseudomonas aeruginosaprecipitins determined by means of crossed immunoelectrophoresis. Scandinavian journal of respiratory diseases, 58 (2), 65-79.

. WELLINGHAUSEN, N., et al. (2005). Superiority of molecular techniques for identification of gram-negative, oxidase-positive rods, including morphologically nontypical Pseudomonas aeruginosa, from patients with cystic fibrosis. Journal of clinical microbiology, 43 (8), 4070-4075.

. ANUJ, Snehal N., et al. (2009). Identification of Pseudomonas aeruginosa by a duplex real-time polymerase chain reaction assay targeting the ecfX and the gyrB genes. Diagnostic microbiology and infectious disease, 63 (2), 127-131.

. YOKOYAMA, Keiko, et al. (2003). Acquisition of 16S rRNAmethylase gene in Pseudomonas aeruginosa. The lancet, 362 (9399), 1888-1893.

. QIN, X., et al. (2003). Use of real-time PCR with multiple targets to identify Pseudomonas aeruginosa and other nonfermenting gram-negative bacilli from patients with cystic fibrosis. Journal of clinical microbiology, 41 (9), 4312-4317.

. MOTOSHIMA, Maiko, et al. (2007). Rapid and accurate detection of Pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis targeting gyrB gene. Diagnostic microbiology and infectious disease, 58 (1), 53-58.

. MELLMANN, A., et al. (2008). Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria. Journal of clinical microbiology, 46 (6), 1946-1954.

. MICEK, S. T., et al. (2005). Pseudomonas aeruginosa bloodstream infection: importance of appropriate initial antimicrobial treatment. Antimicrobial agents and chemotherapy, 49 (4), 1306-1311.

. BERTRAND, X., et al. (2001). Pseudomonas aeruginosa: antibiotic susceptibility and genotypic characterization of strains isolated in the intensive care unit. Clinical microbiology and infection, 7 (12), 706-708.

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Published

2021-09-27

Issue

Vol. 7 No. 3 (2021): EPH - International Journal of Applied Science ( ISSN: 2208-2182 )

Section

Articles

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