DETECTION OF ORAL FUNGAL MICROBIOME (MYCOBIOME) OF DIABETIC PATIENTS IN POLYCLINICS OF BENGHAZI - LIBYA
DOI:
https://doi.org/10.53555/eijas.v7i2.69Keywords:
Diabetics, oral mycobiome, BenghaziAbstract
This study was conducted in Benghazi city, Oral fungal microbiome were isolated from oral cavities of 92 diabetic patients and 40 non diabetic volunteers. Swab taken from their oral cavities then inoculated in Sabauruod Dextrose Agar (SDA) and incubated at 27 °C for two to three days for colony study after that make subcultures using Potato dextrose agar ( PDA ) for microscopic study. Identification of oral fungi shows that Out of 132 subject, 38 shows no fungal growth , 63 samples of candida (53 pure candida and 10 cases candida mixed with other fungi), 7 fungal species other than Candida have been identified with conformation from Assiut University Mycology Centre (AUMC) in Egypt which were : 7 samples of Asperogillus spp (6 in diabetics &1in non diabetics ), 8 samples of Alternaria .alternate (5 in diabetics & 3 in non-diabetics), 3 samples of Fusarium foetens (2 in diabetics & 1in non diabetics ), 4 samples of Cladosporium herbarum. (Diabetics), 2 samples of Stemphylium botryosum (in diabetics), 2 samples of Geosmithia lavendula. (Diabetics), 5 samples of Penicillum crustosum (diabetics). The Candida species identified by Quyfia chest hospital Laboratory (Phoenix 100 Apparatus – BD, USA), .candida species were identified as : Candida albicans (44 cases), Wangilla dermatitidis (Saccaromycets) (7cases Candida dubliniensis ( 4 cases ), Geotricum species ( 4cases ),Candida sake ( 4 cases). Asperogillus species were: 2 samples Asperogillus.nigger, 3 samples A. fumigatus and 2 samples A. flavus.
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